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rabbit anti na v 1 5  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti na v 1 5
    Rabbit Anti Na V 1 5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+na+v+1+5+antibody/Anti-NaV1%2E5+(SCN5A)+(493-511)+Antibody/pmc12785957-182-3-7
    Average 96 stars, based on 137 article reviews
    rabbit anti na v 1 5 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: SNTA1 Gene Rescues Ion Channel Function in Cardiomyocytes Derived from Induced Pluripotent Stem Cells Reprogrammed from Muscular Dystrophy Patients with Arrhythmias
    Article Snippet: Mouse anti-Cardiac Troponin T antibody (1:1000, #Ab10214, Abcam) was used to identify cTnT as the marker for cardiomyocytes. .. Rabbit anti-Na V 1.5 antibody (clone ASC-013, Alomone Labs) was used for Na V 1.5 protein expression (1:500), mouse anti-Kir2.1 antibody (clone N112B/14, University of California at Davis/Nacional Institutes of Health 105 NeuroMab Facility) was used for Kir2.1 protein expression (1:500), mouse anti-Dystrophin (1:000, #D8043, Sigma) was used to detect the Dp427 dystrophin isoform. .. Mouse anti-Actinin antibody (1:1000, #A7811, Sigma) was used to detect Actinin, loading control in total protein analysis.

    Incubation:

    Article Title: Transcription factor Meis1 act as a new regulator of ischemic arrhythmias in mice
    Article Snippet: After quantification with the BCA Protein Assay kit (Beyotime, Shanghai, China), an equal amount of 60 μg protein from each group was fractionated by 8% SDS-PAGE and electro-blotted onto NC membranes (Pall, New York, USA). .. Membranes were incubated with primary antibodies overnight at 4 °C, including rabbit anti-Meis1 antibody (Abcam, UK, Cat#: ab19867, 1:1000), rabbit anti-Na v 1.5 antibody (Alomane laboratory, Israel, Cat#: ASC-005, 1:200), mouse anti-CDC20 antibody (Proteintech Group, America, Cat#: 10252-1-AP, 1:500), mouse anti-Ub antibody (Santa Cruz Biotechnology, Europe, Cat#: sc-8017, 1:1000), mouse anti-His antibody (Santa Cruz Biotechnology, Europe, Cat#: sc-57598, 1:200) and mouse anti-flag antibody (Sigma, USA, Cat#: F1804, 1:1000). ..

    Article Title: Male sex hormone and reduced plakoglobin jointly impair atrial conduction and cardiac sodium currents
    Article Snippet: Freshly isolated murine left atrial cardiac myocytes were plated on 10mm diameter laminin-coated coverslips (Mattek, 35 mm dish, 1.5# coverglass), fixed, permeabilized and blocked. .. Cells were stained with primary rabbit anti-Na v 1.5 antibody (ASC-005, 1:50, Alomone Laboratories, Jerusalem, Israel) and after additional washing and blocking they were incubated in secondary antibody (F(ab’)2-Goat anti-Rabbit IgG, Alexa Fluor 647, A212-56, 1:1000, ThermoFisher Scientific, Waltham, MA, USA). .. Direct stochastic optical reconstruction microscopy (dSTORM) ( ) experiments were performed on a NIKON Eclipse Ti inverted N-STORM microscope equipped with a NIKON APO 100 x 1.49 NA total internal reflection fluorescence (TIRF) oil immersion objective.

    Article Title: Fibroblast Growth Factor 21 Ameliorates Na V 1.5 and Kir2.1 Channel Dysregulation in Human AC16 Cardiomyocytes
    Article Snippet: .. After getting the target bands, the membranes were blocked by 5% filtered nonfat milk for 2 h and then incubated with the indicated primary antibodies overnight at 4°C, including rabbit anti-Na V 1.5 antibody (ASC-005, Alomone Labs, Israel, 1: 200), rabbit anti-Kir2.1 antibody (APC-026, Alomone Labs, Israel, 1: 200) followed by incubation with the corresponding secondary antibody for 1 h at room temperature. .. Protein levels were quantified using the Odyssey Infrared Imaging System (LI-COR, 4607 Superior Street, P.O.

    Article Title: Inter-Regulation of K v 4.3 and Voltage-Gated Sodium Channels Underlies Predisposition to Cardiac and Neuronal Channelopathies
    Article Snippet: .. After washing the beads 4 times with PBS-tween 0.02%, proteins were eluted with the Laemmli sample buffer at 37 °C for 30 min under agitation, separated on a NuPAGE 7% Tris-Acetate gel (Invitrogen), transferred to a nitrocellulose membrane and incubated with primary antibodies: mouse anti-Flag (1:500, Sigma-Aldrich), rabbit anti-Na v 1.5 antibody (1:200, Alomone Labs, Jerusalem, Israel), and mouse anti-transferrin receptor (1:500, Invitrogen) as a loading control. .. Bound antibodies were detected using DyLight-conjugated secondary antibodies (Thermo-Fisher, Waltham, MA, USA), and protein signals were visualized using a Li-Cor Odyssey (Li-Cor Biosciences, Lincoln, NE, USA).

    Staining:

    Article Title: Male sex hormone and reduced plakoglobin jointly impair atrial conduction and cardiac sodium currents
    Article Snippet: Freshly isolated murine left atrial cardiac myocytes were plated on 10mm diameter laminin-coated coverslips (Mattek, 35 mm dish, 1.5# coverglass), fixed, permeabilized and blocked. .. Cells were stained with primary rabbit anti-Na v 1.5 antibody (ASC-005, 1:50, Alomone Laboratories, Jerusalem, Israel) and after additional washing and blocking they were incubated in secondary antibody (F(ab’)2-Goat anti-Rabbit IgG, Alexa Fluor 647, A212-56, 1:1000, ThermoFisher Scientific, Waltham, MA, USA). .. Direct stochastic optical reconstruction microscopy (dSTORM) ( ) experiments were performed on a NIKON Eclipse Ti inverted N-STORM microscope equipped with a NIKON APO 100 x 1.49 NA total internal reflection fluorescence (TIRF) oil immersion objective.

    Blocking Assay:

    Article Title: Male sex hormone and reduced plakoglobin jointly impair atrial conduction and cardiac sodium currents
    Article Snippet: Freshly isolated murine left atrial cardiac myocytes were plated on 10mm diameter laminin-coated coverslips (Mattek, 35 mm dish, 1.5# coverglass), fixed, permeabilized and blocked. .. Cells were stained with primary rabbit anti-Na v 1.5 antibody (ASC-005, 1:50, Alomone Laboratories, Jerusalem, Israel) and after additional washing and blocking they were incubated in secondary antibody (F(ab’)2-Goat anti-Rabbit IgG, Alexa Fluor 647, A212-56, 1:1000, ThermoFisher Scientific, Waltham, MA, USA). .. Direct stochastic optical reconstruction microscopy (dSTORM) ( ) experiments were performed on a NIKON Eclipse Ti inverted N-STORM microscope equipped with a NIKON APO 100 x 1.49 NA total internal reflection fluorescence (TIRF) oil immersion objective.

    other:

    Article Title: Small GTPases SAR1A and SAR1B regulate the trafficking of the cardiac sodium channel Na v 1.5
    Article Snippet: A rabbit anti-Na v 1.5 antibody (ASC-005, Alomone) was used at a dilution factor of 1:1000.

    Membrane:

    Article Title: Inter-Regulation of K v 4.3 and Voltage-Gated Sodium Channels Underlies Predisposition to Cardiac and Neuronal Channelopathies
    Article Snippet: .. After washing the beads 4 times with PBS-tween 0.02%, proteins were eluted with the Laemmli sample buffer at 37 °C for 30 min under agitation, separated on a NuPAGE 7% Tris-Acetate gel (Invitrogen), transferred to a nitrocellulose membrane and incubated with primary antibodies: mouse anti-Flag (1:500, Sigma-Aldrich), rabbit anti-Na v 1.5 antibody (1:200, Alomone Labs, Jerusalem, Israel), and mouse anti-transferrin receptor (1:500, Invitrogen) as a loading control. .. Bound antibodies were detected using DyLight-conjugated secondary antibodies (Thermo-Fisher, Waltham, MA, USA), and protein signals were visualized using a Li-Cor Odyssey (Li-Cor Biosciences, Lincoln, NE, USA).

    Control:

    Article Title: Inter-Regulation of K v 4.3 and Voltage-Gated Sodium Channels Underlies Predisposition to Cardiac and Neuronal Channelopathies
    Article Snippet: .. After washing the beads 4 times with PBS-tween 0.02%, proteins were eluted with the Laemmli sample buffer at 37 °C for 30 min under agitation, separated on a NuPAGE 7% Tris-Acetate gel (Invitrogen), transferred to a nitrocellulose membrane and incubated with primary antibodies: mouse anti-Flag (1:500, Sigma-Aldrich), rabbit anti-Na v 1.5 antibody (1:200, Alomone Labs, Jerusalem, Israel), and mouse anti-transferrin receptor (1:500, Invitrogen) as a loading control. .. Bound antibodies were detected using DyLight-conjugated secondary antibodies (Thermo-Fisher, Waltham, MA, USA), and protein signals were visualized using a Li-Cor Odyssey (Li-Cor Biosciences, Lincoln, NE, USA).



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